TNF-MM20A | 价格

TNF-MM20A-100μg / 询价

TNF-MM20A-500μg / 询价

TNF-MM20A-500μgx2 / 询价

Mouse TNFRSF12A/TWEAKR Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse TNFRSF12A/TWEAKR Protein is expressed from HEK293 with hFc tag at the N-Terminus.
It contains Glu28-Per80 [Accession | Q9CR75].

分子量大小(Molecular Weight)

The protein has a predicted MW of 32.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

纯度(Purity)

>95% as determined by Bis-Tris PAGE
>95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse TNFRSF12A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse TNFRSF12A is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Mouse TNFRSF12A, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.12μg/ml determined by ELISA (QC Test).

SPR Data

Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Mouse TNFRSF12A, hFc Tag with an affinity constant of 0.15 nM as determined in SPR assay (Biacore T200).

背景(Background)

Thyroid cancer (TC) is the most well-known endocrine neoplasia as well as a common malignant tumor in the head and neck.TNFRSF12A expression may be a potential useful prognostic molecular biomarker of bad survival in thyroid cancer, in addition, PPAR signaling pathway, insulin signaling pathway, mTOR signaling pathway may be the key pathway controlled by TNFRSF12A in thyroid cancer. Further experimental ought to be performed to demonstrate the biologic effect of TNFRSF12A.

分子别名(Synonyms)

FGF-inducible 14; Fgfrp2; TNFRSF12A; FN14; CD266; TWEAKR

文献(References)

(1) Wu ZH, Niu X, Wu GH, Cheng Q. Decreased expression of TNFRSF12A in thyroid gland cancer predicts poor prognosis: A study based on TCGA data. Medicine (Baltimore). 2020 Aug 21;99(34):e21882. doi: 10.1097/MD.0000000000021882. PMID: 32846846; PMCID: PMC7447363.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.