Mouse TSLPR Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse TSLPR Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Ala20-Pro232 [Accession | Q8CII9-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 23.6 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse TSLPR is greater than 95% as determined by SEC-HPLC.

背景(Background)

The pro-inflammatory cytokine thymic stromal lymphopoietin (TSLP) plays a pivotal role in the pathophysiology of various allergy disorders that are mediated by type 2 helper T cell (Th2) responses, such as asthma and atopic dermatitis. TSLP forms a ternary complex with the TSLP receptor (TSLPR) and the interleukin-7-receptor subunit alpha (IL-7Rα), thereby activating a signaling cascade that culminates in the release of pro-inflammatory mediators.

分子别名(Synonyms)

TSLP receptor; TSLPR; CRL2; CRLF2Y; CRLF2; IL-XR; p2RY8/CRLF2 fusion; CRLF2

文献(References)

(1) Van Rompaey D, Verstraete K, Peelman F, Savvides SN, Augustyns K, Van Der Veken P, De Winter H. Virtual screening for inhibitors of the human TSLP:TSLPR interaction. Sci Rep. 2017 Dec 8;7(1):17211. doi: 10.1038/s41598-017-17620-7. Erratum in: Sci Rep. 2018 Apr 24;8(1):6673. PMID: 29222519; PMCID: PMC5722893.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.