Biotinylated Human CD155/PVR Protein (Primary Amine Labeling)

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human CD155/PVR Protein (Primary Amine Labeling) is expressed from Expi293 with hFc tag at the C-terminal. It contains Trp21-Asn343.[Accession | P15151-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per ug by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human CD155 (Primary Amine Labeling) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human CD155 (Primary Amine Labeling) is greater than 95% as determined by SEC-HPLC.

Blocking Data

Serial dilutions of Anti-TIGIT Antibody were added into Human TIGIT, His Tag : Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.46μg/ml.

背景(Background)

CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.

分子别名(Synonyms)

CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946

文献(References)

(1)Yu X , Harden K , C Gonzalez L , et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57.

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