货号价格

EGF-HE001-100μg / 询价

EGF-HE001-500μg / 询价

EGF-HE001-500μgx2 / 询价

Human EGF Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human EGF Protein is expressed from E.coli without tag.
It contains Asn971-Arg1023 [Accession | P01133-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 5.6 kDa same as Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 0.2EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human EGF is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human EGF, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.82μg/ml determined by ELISA (QC Test).

SPR Data

Human EGFR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human EGF, No Tag with an affinity constant of 0.18 nM as determined in SPR assay (Biacore T200).

Cell Based Assay

Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1 - 0.2 ng/mL (QC Test).

背景(Background)

The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.

分子别名(Synonyms)

HOMG4; EGF; URG

文献(References)

(1)Normanno N, Bianco C, De Luca A, Salomon DS. The role of EGF-related peptides in tumor growth. Front Biosci. 2001 May 1;6:D685-707. doi: 10.2741/normano. PMID: 11333208.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.