EGF-HE001-100μg / 询价
EGF-HE001-500μg / 询价
EGF-HE001-500μgx2 / 询价
Human EGF Protein
Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023 [Accession | P01133-1].
The protein has a predicted MW of 5.6 kDa same as Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 0.2EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human EGF is greater than 95% as determined by SEC-HPLC.
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1 - 0.2 ng/mL (QC Test).
Immobilized Human EGF, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.56μg/ml determined by ELISA (QC Test).
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, No Tag with an affinity constant of 1.33 nM as determined in SPR assay (Biacore T200).
The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
HOMG4; EGF; URG
(1)Normanno N, Bianco C, De Luca A, Salomon DS. The role of EGF-related peptides in tumor growth. Front Biosci. 2001 May 1;6:D685-707. doi: 10.2741/normano. PMID: 11333208.
