FRN-HM401B-100μg / 询价
FRN-HM401B-500μg / 询价
FRN-HM401B-500μgx2 / 询价
Biotinylated Human FcRn Protein
Recombinant Biotinylated Human FcRn Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala24-Ser297(FCGRT)&Ile21-Met119(B2M) [Accession | P55899(FCGRT)&P61769((B2M)].
The protein has a predicted MW of 46.5 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human FcRn is greater than 95% as determined by SEC-HPLC.
Biotinylated Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 0.557 uM asdetermined in SPR assay (Biacore T200). (QC Test)
Loaded Biotinylated Human FcRn, His-Avi Tag on Streptavidin-Biosensor can bind Human Serum Albumin, Llama Fc Tag with an affinity constant of 24.7 nM as determined in BLI assay (Gator® Prime).
The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.
FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
(1) Kiessling P , Lledo-Garcia R , Watanabe S , et al. The FcRn inhibitor rozanolixizumab reduces human serum IgG concentration: A randomized phase 1 study[J]. Science translational medicine, 2017, 9(414):eaan1208.
