FSF-MM218 | 价格

FSF-MM218-100μg / 询价

FSF-MM218-500μg / 询价

FSF-MM218-500μgx2 / 询价

Mouse GITR Ligand/TNFSF18 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse GITR Ligand/TNFSF18 Protein is expressed from HEK293 with hFc tag at the N-Terminus.
It contains Thr47-Ser173 [Accession | Q7TS55].

分子量大小(Molecular Weight)

The protein has a predicted MW of 41.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse GITR Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse GITR Ligand is greater than 95% as determined by SEC-HPLC.

背景(Background)

Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.

分子别名(Synonyms)

TNFSF18; AITRL; TL6; GITRL; GITR Ligand

文献(References)

(1)Richards DM, Marschall V, Billian-Frey K, et al. HERA-GITRL activates T cells and promotes anti-tumor efficacy independent of FcγR-binding functionality. J Immunother Cancer. 2019;7(1):191. Published 2019 Jul 19. doi:10.1186/s40425-019-0671-4

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.