ILR-HM212 | 价格

ILR-HM212-100μg / 询价

ILR-HM212-500μg / 询价

ILR-HM212-500μgx2 / 询价

Human IL-12 R beta 1/CD212 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-12 R beta 1/CD212 Protein is expressed from HEK293 with hFc tag at the C-terminus.
It contains Cys24-Glu540 [Accession | P42701-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 83.85 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human IL-12 R beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human IL-12B, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA.

背景(Background)

Human IL12RB1 is an autosomal gene that is essential for mycobacterial disease resistance and T cell differentiation. Lung and T cell IL12RB1 expression is allele-biased, and the extent to which cells express one IL12RB1 allele is unaffected by activation. Furthermore following its expression the IL12RB1 pre-mRNA is processed into either IL12RB1 Isoform 1 (IL12Rβ1, a positive regulator of IL12 responsiveness) or IL12RB1 Isoform 2 (a protein of heretofore unknown function).

分子别名(Synonyms)

IL-12R-beta-1; IL-12RB1; IL12R; IL12RB; CD212; IL-12R subunit beta-1; IL-12R-BETA1; MGC34454;IL-12R-β-1; IL-12R subunit β-1; IL-12R-β1

文献(References)

(1) Reeme AE, Claeys TA, Aggarwal P, Turner AJ, Routes JM, Broeckel U, Robinson RT. Human IL12RB1 expression is allele-biased and produces a novel IL12 response regulator. Genes Immun. 2019 Mar;20(3):181-197. doi: 10.1038/s41435-018-0023-2. Epub 2018 Mar 30. PMID: 29599514; PMCID: PMC6165718.