RNF-HM143 | 价格

RNF-HM143-100μg / 询价

RNF-HM143-500μg / 询价

RNF-HM143-500μgx2 / 询价

Human RNF43 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human RNF43 Protein is expressed from HEK293 with His tag at the C-terminus.
It contains Gly24-Tyr197 [Accession | Q68DV7-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 20.14 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human RNF43 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC.

SPR Data

Human R-Spondin 3, His Tag immobilized on CM5 Chip can bind Human RNF43, His Tag with an affinity constant of 0.255 μM as determined in SPR assay (Biacore T200).

背景(Background)

RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.

分子别名(Synonyms)

E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43

文献(References)

(1) Serra S, Chetty R. Rnf43. J Clin Pathol. 2018 Jan;71(1):1-6. doi: 10.1136/jclinpath-2017-204763. Epub 2017 Oct 10. PMID: 29018044.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.