货号价格

RNF-HM234-100μg / 询价

RNF-HM234-500μg / 询价

RNF-HM234-500μgx2 / 询价

Human RNF43 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human RNF43 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gly24-Tyr197.[Accession | Q68DV7-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human RNF43 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human R spondin 1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4μg/ml determined by ELISA.

背景(Background)

RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.

分子别名(Synonyms)

E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43

文献(References)

(1) Serra S, Chetty R. Rnf43. J Clin Pathol. 2018 Jan;71(1):1-6. doi: 10.1136/jclinpath-2017-204763. Epub 2017 Oct 10. PMID: 29018044.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.