SRP-HM4V5-100μg / 询价
SRP-HM4V5-500μg / 询价
SRP-HM4V5-500μgx2 / 询价
Human SIRP alpha V5 Protein
Recombinant Human SIRP alpha V5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Arg370(S42F) [Accession | P78324 variant 5].
The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 24 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human SIRP alpha V5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human SIRP alpha V5 is greater than 95% as determined by SEC-HPLC.
Immobilized Human SIRP alpha V5, His Tag at 2μg/ml (100μl/Well).Dose response curve for Human CD47, hFc Tag with the EC50 of 9.7ng/ml determined by ELISA (QC Test).
Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.
CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA
(1)Weiskopf K , Ring A M , Ho C C M , et al. Engineered SIRP? Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies[J]. Science, 2013, 341(6141):88-91.
