Biotinylated Human CD47 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human CD47 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains GIn19-Pro139.[Accession | Q08722-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 16.63 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human CD47 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human SIRP alpha, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human CD47, His Tag with the EC50 of 0.48μg/ml determined by ELISA.

背景(Background)

CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.

分子别名(Synonyms)

CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6

文献(References)

(1) Weiskopf K , Ring A M , Ho C C M , et al. Engineered SIRP? Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies[J]. Science, 2013, 341(6141):88-91.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.