货号价格

TG1-HM10MB-100μg / 询价

TG1-HM10MB-500μg / 询价

TG1-HM10MB-500μgx2 / 询价

Biotinylated Human Mature TGF beta 1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human Mature TGF beta 1 Protein is expressed from HEK293 with Avi tag at the C-Terminus. It contains Ala279-Ser390.[Accession | P01137]

分子量大小(Molecular Weight)

The protein has a predicted MW of 13.2 kDa. Due to glycosylation, the protein migrates to 15-18 kDa under reduced (R) condition and 26-30 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 50mM Glycine, 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCl.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human Mature TGF beta 1 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

ELISA Data

Immobilized Human TGF-beta RII, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 9.9ng/ml determined by ELISA.

背景(Background)

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition.Transforming growth factor beta-1 is multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration.

分子别名(Synonyms)

CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1

文献(References)

(1)Robertson I B , Rifkin D B. Regulation of the Bioavailability of TGF-β and TGF-β-Related Proteins[J]. Cold Spring Harbor Perspectives in Biology, 2016, 8(6):a021907.