TG2-HM00MB | 价格

TG2-HM00MB-100μg / 询价

TG2-HM00MB-500μg / 询价

TG2-HM00MB-500μgx2 / 询价

Biotinylated Human Mature TGF beta 2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human Mature TGF beta 2 Protein is expressed from HEK293 with Avi tag at the C-Terminus.
It contains Ala303-Ser414 [Accession | P61812-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 14.53 kDa same as Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCl.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human Mature TGF beta 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.17μg/ml determined by ELISA (QC Test).

背景(Background)

Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs.

分子别名(Synonyms)

TGFB2; TGFBR2; TGFR2; TbetaR-II; TGFβR2; BB105277; TGF-beta 2; TβR-II; TGF-β 2

文献(References)

Yao K, Tan J, Gu WZ, Ye PP, Wang KJ. Reactive oxygen species mediates the apoptosis induced by transforming growth factor beta(2) in human lens epithelial cells. Biochem Biophys Res Commun. 2007 Mar 2;354(1):278-83. doi: 10.1016/j.bbrc.2006.12.198. Epub 2007 Jan 10. PMID: 17217916.